Chemical Constituents of Anaphalis hancockii Maxim

Twenty-seven compounds were isolated from Anaphalis hancockii Maxim in Qinghai Province, China, including 7 isobenzofuranones (1-7), 8 flavonoids (8-15), and 12 other compounds (16-27). Compound 1 is a new isobenzofuranone. The structures of the compounds were determined by analyzing their HRESIMS, 1D- and 2D-nuclear magnetic resonance (NMR), electronic circular dichroism (ECD) and comparing them to the reported data. This study is the first report of A. hancockii Maxim's chemical composition, a perennial herb belonging to the genus Anaphalis. Isobenzofuranones are the main bioactive constituents of the genus Anaphalis; thus, seven isobenzofuranones (1-7) were assessed for their antibacterial and antichlamydial activities. Compound 6 showed moderate activity against six different pathogenic fungi. The result suggested that Compound 5 might be a promising class of therapeutic agents for chlamydial infections.