Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Volatilization01:10

Volatilization

547
Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...
547

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Repurposing the liquid-based Pap test for the detection of ovarian cancer protein biomarkers.

Clinical proteomics·2026
Same author

Characterization of Gait Kinematics and Muscle Function in Becker Muscular Dystrophy Pigs: a pilot study.

bioRxiv : the preprint server for biology·2025
Same author

The acute effects of alpha-lactalbumin intake on tryptophan metabolites and mood in older adults with Mild Cognitive Impairment.

Nutritional neuroscience·2025
Same author

Alterations of the skeletal muscle nuclear proteome after acute exercise reveals a posttranscriptional influence.

American journal of physiology. Cell physiology·2025
Same author

Muscle metabolic resilience and enhanced exercise adaptation by Esr1-induced remodeling of mitochondrial cristae-nucleoid architecture in males.

Cell reports. Medicine·2025
Same author

Presence of chronic morbidities alters skeletal muscle health and amino acid kinetics in mild cognitive impairment.

Journal of Alzheimer's disease : JAD·2025

Related Experiment Video

Updated: Sep 14, 2025

Breath Collection from Children for Disease Biomarker Discovery
06:09

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

7.0K

Volatile organic compound and proteomics data from the same exhaled breath condensate sample.

Shannon E Schrader1, Joshua R Hansen1, Isabelle O'Bryon1

  • 1Chemical and Biological Signatures Group, Pacific Northwest National Laboratory, Richland, WA 99352, United States of America.

Journal of Breath Research
|July 23, 2025
PubMed
Summary

Analyzing volatile organic compounds (VOCs) and proteins from single exhaled breath condensate (EBC) samples simplifies diagnostics. Thin film-solid phase microextraction (TF-SPME) for VOCs did not impact protein detection in EBC.

Keywords:
exhaled breath condensateliquid chromatography-tandem mass spectrometryproteomicstwo-dimensional gas chromatography-mass spectrometryvolatile organic compounds

More Related Videos

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.2K
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.0K

Related Experiment Videos

Last Updated: Sep 14, 2025

Breath Collection from Children for Disease Biomarker Discovery
06:09

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

7.0K
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.2K
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.0K

Area of Science:

  • Biomarker Discovery
  • Analytical Chemistry
  • Multi-omics

Background:

  • Exhaled breath condensate (EBC) is a non-invasive source for disease diagnostics.
  • Analyzing multiple analytes from a single EBC sample streamlines the diagnostic process.
  • Current methods often require separate samples for different analyses, increasing patient burden.

Purpose of the Study:

  • To investigate the feasibility of analyzing both volatile organic compounds (VOCs) and proteins from the same EBC sample.
  • To determine if thin film-solid phase microextraction (TF-SPME) for VOC analysis affects subsequent proteomic analysis.
  • To advance multi-omics breath analysis from a single sample.

Main Methods:

  • EBC samples were collected and divided into two groups: TF-SPME for VOC analysis and non-TF-SPME for proteomic analysis.
  • VOCs were analyzed using two-dimensional gas chromatography-mass spectrometry (GC x GC-MS).
  • Proteins were analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS).

Main Results:

  • 184 VOCs were found to be more abundant in EBC samples compared to controls.
  • No significant difference in the number of detected proteins between TF-SPME and non-TF-SPME groups.
  • 144 out of 206 unique proteins were detected in both sample types, indicating TF-SPME does not hinder proteomic analysis.

Conclusions:

  • TF-SPME is a suitable method for VOC analysis without compromising proteomic integrity in EBC.
  • This study supports the development of multi-omics analysis from single EBC samples.
  • This approach offers a more efficient and patient-friendly diagnostic strategy.