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Related Experiment Video

Updated: Sep 14, 2025

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
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Altered platelet lipidome in bleeding patients with unexplained platelet function defects.

Bianca De Jonckheere1, Dino Mehic2, Dominik Kopczynski3

  • 1Institute of Analytical Chemistry, University of Vienna, Vienna, Austria; Vienna Doctoral School in Chemistry, University of Vienna, Vienna.

Haematologica
|July 24, 2025
PubMed
Summary
This summary is machine-generated.

Platelet lipid profiles reveal sex-specific differences in patients with unexplained bleeding disorders. Lipidomic analysis offers a diagnostic tool for platelet dysfunction and identifies potential therapeutic targets like triacylglycerols and lysophosphatidylethanolamines.

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Area of Science:

  • Hematology
  • Lipidomics
  • Biochemistry

Background:

  • Platelet function defects (PFD) in mild to moderate bleeding disorders (MBD) often have elusive underlying mechanisms.
  • Lipids play a crucial role in platelet signaling, suggesting their potential as diagnostic markers.

Purpose of the Study:

  • To investigate platelet lipid profiles in MBD patients with unexplained PFD.
  • To identify potential diagnostic and mechanistic insights from platelet lipidomics.

Main Methods:

  • Analysis of platelet lipidomes in 27 MBD patients with unexplained PFD and 19 controls using a standardized lipidomics workflow.
  • Correlation of lipid alterations with platelet aggregation and responses to stimuli (ADP, TRAP-6) in light transmission aggregometry (LTA).

Main Results:

  • Sex-specific lipid shifts were observed in patients, with greater deviations in females.
  • Lipid alterations correlated with impaired platelet aggregation and predicted responses to ADP and TRAP-6.
  • Specific changes in females included upregulated polyunsaturated triacylglycerols (PUFA-TGs) and acylcarnitines (CARs), and reduced lysophosphatidylethanolamines (LPEs).

Conclusions:

  • Lipidomic profiling is a promising diagnostic approach for unexplained platelet dysfunction.
  • Triacylglycerols (TGs), CARs, and LPEs are potential therapeutic targets for bleeding disorders.
  • Further research into lipid-driven platelet regulation can advance personalized treatments for MBD.