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Site-2 protease-like protein drives organic solvent tolerance in Rhodococcus ruber.

Jinfang Wu1, Zhonghao Wu1, Ren Peng1

  • 1College of Life Science, Jiangxi Normal University, Nanchang 330022, China.

Journal of Microbiological Methods
|July 24, 2025
PubMed
Summary

Overexpressing a Site-2 protease-like protein (S2plp) in Rhodococcus ruber boosts its tolerance to organic solvents. This genetic enhancement improves bacterial resilience and potential for biocatalysis and bioremediation applications.

Keywords:
Rhodococcus ruberSite-2 proteaseSolvents toleranceTranscriptomic analysis

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Rhodococcus ruber is a bacterium with potential applications in biocatalysis and bioremediation.
  • Enhancing organic solvent tolerance is crucial for industrial applications of microorganisms.

Purpose of the Study:

  • To investigate the role of Site-2 protease-like protein (S2plp) in Rhodococcus ruber's organic solvent tolerance.
  • To identify molecular mechanisms underlying enhanced solvent tolerance.

Main Methods:

  • Genetic engineering of Rhodococcus ruber SD3 to overexpress S2plp.
  • Exposure of engineered strains to various organic solvents.
  • Transcriptomic analysis to identify differentially expressed genes.
  • KEGG pathway analysis to understand molecular functions.

Main Results:

  • Engineered S2plp overexpression significantly enhanced organic solvent tolerance in Rhodococcus ruber.
  • Transcriptomic analysis revealed upregulation of 13 genes, including those involved in DNA repair.
  • KEGG analysis indicated that S2plp overexpression activates DNA repair pathways.

Conclusions:

  • S2plp is a novel molecular target for improving organic solvent tolerance in Rhodococcus ruber.
  • Enhanced DNA repair mechanisms contribute to increased solvent resilience.
  • This study advances the potential of Rhodococcus ruber in biocatalysis and bioremediation.