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A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
Published on: October 2, 2012
Site-2 protease-like protein drives organic solvent tolerance in Rhodococcus ruber
Jinfang Wu1, Zhonghao Wu1, Ren Peng1
1College of Life Science, Jiangxi Normal University, Nanchang 330022, China.
Abstract:
Engineered overexpression of a Site-2 protease-like protein (S2plp) in Rhodococcus ruber SD3 significantly enhanced organic solvent tolerance. Through electro-transformation with the recombinant plasmid pNV18-s2plp-s2plp, we generated a strain exhibiting superior growth under multiple organic solvent stresses. Transcriptomic analysis identified 13 significantly upregulated genes, including those encoding aminoglycoside O-phosphotransferase, replication initiation protein, two M50 family metallopeptidases, transposase, SMC family ATPase, ATP-dependent helicase, three hypothetical proteins, and three small RNAs (sRNAs). KEGG analysis linked S2plp overexpression to enhanced DNA repair mechanisms, directly contributing to organic solvent tolerance. These results establish S2plp as a novel molecular target for boosting solvent resilience in this important bacterium, advancing its potential in biocatalysis and bioremediation.
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