Related Experiment Video
Updated: May 10, 2026
An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Published on: April 9, 2015
Isolation and identification of bacteriophage against Escherichia coli ATCC 25922 and their biofilm Inhibition
Lakshminarayanan Sivakumar1, Jeya Vignesh John Durai Kumar1, Subadarshini Madhavan1
1Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, 603203, Tamil Nadu, India.
Abstract:
Bacterial biofilm development plays a crucial role in causing serious health concerns in the medical sector. The adhesion of germs on living and non-living surfaces can lead to damage as well as diseases in humans, and other animals. To address this problem, researchers focus exclusively on bacteriophage therapy. In the present study, lytic phage with 1010 PFU/mL was isolated from hospital sewage samples by targeting Escherichia coli (ATCC 25922). The morphological study of the isolated phage was examined under high-resolution transmission electron microscopy (HR-TEM) by using 2% of uranyl acetate as a negative staining, which revealed that the isolated phage belonged to the Myoviridae family. Bacterial turbidity assay showed reduced optical density (OD) in 0.01 multiplicity of infection (MOI) and no growth in 0.1 and 1.0 MOI phage concentration which was compared with control (without phage-treated E. coli cells). The phage-host interaction was investigated using an HR-TEM after treating the phage for 30 min, which showed the lytic cycle of the phage. Further, the E. coli biofilm was assessed using a microplate reader after 24 and 48 h on 96 titer plates with crystal violet staining to confirm the inhibition efficiency of phage-treated biofilm quantitatively (83%). Then the compound and fluorescence microscopy were used for qualitative measurement of inhibition activity. Further, the biofilm formation of E. coli on a coverslip surface was analysed using scanning electron microscopy, comparing samples treated with 0.1 MOI of phage to untreated controls. About 10% of countable cells only present over the phage-treated surface (90% of biofilm inhibition). The outcome of this study revealed that phage treatment effectively suppressed the E. coli biofilm development, and it can be used as a sustainable and eco-friendly approach for the control of biofilm formation on surfaces specifically in hospital environments.
Insights
Bacteriophage therapy effectively inhibited Escherichia coli biofilm formation. This sustainable approach offers a promising solution for controlling harmful bacterial growth in healthcare settings.
Area of Science:
- Microbiology
- Biotechnology
- Infectious Diseases
Background:
- Bacterial biofilms pose significant health risks in medical settings, causing damage and disease.
- Controlling biofilm formation is critical for preventing infections and improving patient outcomes.
- Bacteriophage therapy is an emerging strategy to combat antibiotic-resistant bacteria and biofilms.
Purpose of the Study:
- To isolate and characterize a lytic bacteriophage targeting Escherichia coli.
- To evaluate the efficacy of bacteriophage therapy in inhibiting E. coli biofilm development.
- To explore the potential of bacteriophages as a sustainable and eco-friendly anti-biofilm agent.
Main Methods:
- Isolation of lytic phage from hospital sewage targeting E. coli (ATCC 25922).
- Morphological characterization using high-resolution transmission electron microscopy (HR-TEM).
- Assessment of biofilm inhibition using microplate reader assays, crystal violet staining, fluorescence microscopy, and scanning electron microscopy (SEM).
Main Results:
- A Myoviridae family lytic phage was isolated and confirmed to exhibit a lytic cycle against E. coli.
- Phage treatment significantly reduced bacterial turbidity and E. coli biofilm formation.
- Quantitative analysis showed up to 83% inhibition of E. coli biofilm, with SEM revealing 90% inhibition.
Conclusions:
- Phage treatment effectively suppresses E. coli biofilm development.
- Bacteriophage therapy presents a sustainable and eco-friendly alternative for controlling biofilm formation.
- This approach holds promise for applications in hospital environments to mitigate infection risks.
More Related Videos
07:22Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
Published on: August 19, 2021
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
