New way to isolate cultured cell exosomes

Exosomes are one of the extracellular vesicles that are secreted by almost all cell types and body fluids. Because they are nanosized (30-200 nm), they can be used as natural nanovesicles. Exosomes have recently been preferred for their low immunogenicity and toxicity features for cell-free therapy, nano-drug carriers, and regenerative medicine. Rapid and appropriate exosome isolation has become increasingly critical due to its extensive application area. In this study, we isolated the MCF-7 cell exosomes using a biological membrane that works for nanoparticle isolation. Our results showed that the number of exosomes was 2 × 106 particles per ml in the cell line media, with a peak size of 110 nm. The proposed technique has features such as simplifying the operative procedures, low cost, and high efficiency. In addition, this technique did not use high-cost reactants, and it was not time-consuming. Additionally, no further procedure was necessary, and the amount of hand manipulation was minimal.