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Updated: Sep 13, 2025
Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry GC-MS-based Metabolomics
Published on: June 6, 2018
Protocol to quantify compound retention in Drosophila melanogaster for the pharmacokinetic study of orally
Nadiia Sadova1, Bernhard Blank-Landeshammer2, Julian Weghuber1
1Center of Excellence Food Technology and Nutrition, University of Applied Sciences Upper Austria, Stelzhamerstrasse 23, Wels 4600, Austria; FFoQSI GmbH-Austrian Competence Centre for Feed and Food Quality, Safety and Innovation, Technopark 1D, Tulln 3430, Austria.
Abstract:
Drosophila melanogaster is a valuable tool for intestinal function research. Here, we present a compound retention assay (CORE) to quantify the orally administered compounds that were absorbed, metabolized, or accumulated in the tissues (for example, fat) of D. melanogaster. We describe steps for preparing flies and media, compound administration, and sample collection. We then detail procedures for determining concentrations and calculating CORE values. This protocol circumvents the limitations of bioavailability research in the fruit fly, such as administering precise compound amounts. For complete details on the use and execution of this protocol, please refer to Sadova et al.1.
