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Biotin/sulfasalazine combination therapy alleviates acetic acid-induced ulcerative colitis in rats via modulation of

Sahar A Helmy1, Mahmoud M Samaha2, Al Shaima G Abd El Salam3

  • 1Department of Biochemistry, Faculty of Pharmacy, Mansoura University, P. O. Box 35516, Mansoura, Egypt. saharabdelgawad@mans.edu.eg.

Scientific Reports
|July 27, 2025
PubMed

Insights

Sulfasalazine (SLZ) combined with Biotin effectively treats ulcerative colitis (UC) in rats. This combination therapy reduced inflammation and restored colon health, offering a promising new treatment for UC.

Area of Science:

  • Gastroenterology
  • Pharmacology
  • Inflammation Research

Background:

  • Ulcerative colitis (UC) is a chronic inflammatory bowel disease with limited effective treatments.
  • Novel therapeutic strategies are needed to manage UC effectively.

Purpose of the Study:

  • To investigate the therapeutic potential of combining sulfasalazine (SLZ) and Biotin for acetic acid (AA)-induced UC in a rat model.
  • To elucidate the underlying mechanisms of action for the SLZ/Biotin combination therapy.

Main Methods:

  • Ulcerative colitis was induced in rats using intra-rectal acetic acid injection.
  • Rats were treated with SLZ, Biotin, or a combination of both for 8 days.
  • Disease activity, colonic pathology, serum markers (LDH, CRP), redox status (MDA, NO, GSH), and inflammatory markers (S1P, S1PR1, IL-23, STAT3, P-STAT3, COX-2, NF-κB) were assessed.

Main Results:

  • SLZ/Biotin combination therapy significantly attenuated UC symptoms, reducing colonic damage and disease activity index.
  • The treatment restored redox balance in the colon by modulating MDA, NO, and GSH levels.
  • Combination therapy markedly decreased colonic levels of S1P, S1PR1, IL-23, STAT3, P-STAT3, COX-2, and NF-κB, indicating reduced inflammation.

Conclusions:

  • Biotin as an add-on to SLZ demonstrates significant therapeutic efficacy in an experimental model of UC.
  • The combination therapy alleviates UC by modulating the S1P/S1PR1/NF-kB/IL-23/STAT3 inflammatory pathway and inhibiting COX-2.
  • This combination represents a promising therapeutic approach for managing ulcerative colitis.
Keywords:
BiotinIL-23S1PR1STAT3SulfasalazineUlcerative colitis

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