Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

5.0K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Ink to Mockup: An Integrated Study of Historical Gall Ink Formulations and Their Impact on Paper.

ChemPlusChem·2026
Same author

NMR-based urinary biomarkers in pediatric primary mitochondrial disorders and chronic kidney disease: shared mitochondrial dysfunction, diverging biosignatures.

Metabolomics : Official journal of the Metabolomic Society·2026
Same author

Injectable and self-healable supramolecular hydrogels assembled by quaternised chitosan/alginate polyelectrolyte complexation for sustained drug delivery and cell encapsulation.

Biomaterials science·2025
Same author

Understanding Hypertension: A Metabolomic Perspective.

Biology·2025
Same author

Advances in abiotic tissue-based biomaterials: A focus on decellularization and devitalization techniques.

Materials today. Bio·2025
Same author

Impact of High-Fat Diet-induced Metabolic Dysfunction-associated Steatotic Liver Disease on Heart, Kidney, and Skeletal Muscle Metabolomes in Wild-Type Mice.

Journal of proteome research·2025

Related Experiment Video

Updated: Sep 13, 2025

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

9.5K

Exploring In Vitro Mesenchymal Stem Cell Osteodifferentiation via Vibrational Microspectroscopy: A Review.

Daniela S Bispo1, Inês C R Graça1, João A Rodrigues1

  • 1Department of Chemistry, CICECO - Aveiro Institute of Materials, Campus Universitário de Santiago, University of Aveiro, 3810-193, Aveiro, Portugal.

Stem Cell Reviews and Reports
|July 27, 2025
PubMed
Summary

Vibrational microspectroscopy, particularly Raman, offers a label-free method to study mesenchymal stem cells (MSCs) osteogenic differentiation. This technique aids in understanding bone formation and selecting suitable MSCs for clinical applications.

Keywords:
FTIRMesenchymal stem cellsMicroscopyOsteogenic differentiationRamanVibrational spectroscopy

More Related Videos

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
08:11

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Published on: January 31, 2019

11.4K
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

5.8K

Related Experiment Videos

Last Updated: Sep 13, 2025

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

9.5K
Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
08:11

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Published on: January 31, 2019

11.4K
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

5.8K

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Cell Biology

Background:

  • Mesenchymal stem cells (MSCs) are crucial for bone regeneration.
  • Understanding their osteogenic differentiation is key for bone fabrication.
  • Vibrational microspectroscopy provides molecular insights into this process.

Purpose of the Study:

  • To review the application of vibrational microspectroscopy (IR and Raman) in studying MSC osteogenic differentiation.
  • To highlight advancements in characterizing MSCs and their extracellular matrix.
  • To discuss the potential of these techniques for clinical translation.

Main Methods:

  • Application of infrared (IR) and Raman microspectroscopy.
  • Focus on bone marrow, dental/oral, and adipose-derived MSCs.
  • Analysis of single cells and extracellular matrix at the molecular level.
  • Examination of spectral band positions, linewidths, shapes, and ratios.
  • Use of multivariate analysis and machine learning.
  • Exploration of advanced IR and Raman techniques for higher sensitivity and resolution.

Main Results:

  • Raman microspectroscopy is predominantly used.
  • Studies focus on mineralization maturity, mineral aggregates, and collagen interactions.
  • Time-dependent changes in protein structure and ECM components are analyzed.
  • Spectral band analysis and ratios effectively assess mineral evolution and matrix interactions.
  • Multivariate analysis and machine learning enhance spectral variation detection.

Conclusions:

  • Vibrational microspectroscopy is a powerful, label-free tool for studying MSC osteogenic differentiation.
  • It enables detailed molecular characterization of MSCs and their matrix.
  • The non-invasive nature facilitates rapid donor selection for clinical applications.
  • Further advancements promise improved sensitivity and resolution for bone tissue engineering.