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A Modified Bioceramic Sealer with Dual Antibacterial Mechanisms
Bashayer Baras1, Amal Almohaimede2, Yara Alshibani3
1Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh 11432, Saudi Arabia.
Bioengineering (Basel, Switzerland)
|July 29, 2025
Summary
Adding silver nanoparticles (NAg) and dimethylaminohexadecyl methacrylate (DMAHDM) to root canal sealers significantly enhanced their antibacterial effect against Enterococcus faecalis biofilms. This combination maintained acceptable physical properties for endodontic applications.
Area of Science:
- Biomaterials Science
- Microbiology
- Endodontics
Background:
- Root canal sealers are crucial for endodontic success.
- Enhancing antibacterial properties of sealers is an ongoing research area.
- Enterococcus faecalis is a common pathogen in persistent endodontic infections.
Purpose of the Study:
- To evaluate the antibacterial efficacy of silver nanoparticles (NAg) and dimethylaminohexadecyl methacrylate (DMAHDM) in EndoSequence Bioceramic (BC) sealer.
- To assess the impact of these additives on the physical properties of the sealer.
- To investigate the antibiofilm activity against Enterococcus faecalis.
Main Methods:
- Four groups of sealers were prepared: control, NAg-modified, DMAHDM-modified, and combined NAg and DMAHDM modified.
- Physical properties (flowability, film thickness) were tested according to ISO standards.
- Antibacterial activity was assessed against Enterococcus faecalis biofilms on dentin discs by measuring colony-forming unit (CFU) counts.
Main Results:
- All tested sealer formulations met ISO requirements for flowability and film thickness.
- The combination of 0.15% NAg and 5% DMAHDM significantly reduced Enterococcus faecalis CFU counts by approximately 5 logs.
- Individual additives also showed some reduction in CFU counts, but the combination was most effective.
Conclusions:
- The combination of silver nanoparticles and DMAHDM presents a promising strategy for developing advanced endodontic sealers.
- These enhanced sealers exhibit potent antimicrobial properties against Enterococcus faecalis biofilms.
- The tested concentrations maintain acceptable physical characteristics essential for clinical application.
Keywords:
DMAHDMantibacterial agentsendodonticsinnovative materialsphysical propertiessilver nanoparticlesMore Related Videos
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