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Related Experiment Video

Updated: Sep 13, 2025

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
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Component-Resolved and Multiplex-Specific IgE Diagnostics: Utility in Anaphylaxis and Beyond.

Mirjana Turkalj1,2,3, Ivana Banić4, Gordana Fressl Juroš5

  • 1Department of Allergy and Clinical Immunology, Srebrnjak Children's Hospital, HR-10000 Zagreb, Croatia.

Children (Basel, Switzerland)
|July 29, 2025
PubMed
Summary

Component-resolved diagnostics (CRD) and multiplex assays enhance the diagnosis of allergic diseases and anaphylaxis. These advanced tools improve risk assessment for severe reactions and guide personalized treatment plans.

Keywords:
allergen-specific immunoglobulin E (IgE)anaphylaxiscomponent-resolved diagnosticsmolecular diagnostics in allergymultiplex IgE assayssingleplex sIgE assay

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Area of Science:

  • Allergy and Immunology
  • Diagnostic Medicine

Background:

  • Allergic disease and anaphylaxis diagnosis is complex, involving various in vitro and in vivo tests.
  • Test selection depends on the suspected allergic reaction mechanism.

Purpose of the Study:

  • To review the advantages of component-resolved diagnostics (CRD) and multiplex assays in clinical practice.
  • To highlight their role in diagnosing anaphylaxis and guiding immunotherapy.

Main Methods:

  • Review of current literature on diagnostic tools for allergic diseases.
  • Focus on component-resolved diagnostics (CRD) and multiplex-specific immunoglobulin E (IgE) platforms.

Main Results:

  • CRD has improved the depiction of sensitization profiles and assessment of clinically relevant allergens.
  • Multiplex platforms aid in selecting patients at risk for anaphylaxis and for allergen immunotherapy, including oral immunotherapy.

Conclusions:

  • CRD and multiplex assays are crucial for diagnosing anaphylaxis, especially with unclear triggers or multiple suspected allergens.
  • These diagnostics facilitate personalized management plans and may reduce the financial burden of anaphylaxis.