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In Silico Proteomic Profiling and Bioactive Peptide Potential of Rapeseed Meal
Katarzyna Garbacz1,2, Jacek Wawrzykowski3, Michał Czelej1,2
1Biolive Innovation Sp. z.o.o., Dobrzańskiego 3, 20-262 Lublin, Poland.
Foods (Basel, Switzerland)
|July 29, 2025
Summary
Rapeseed meal proteins can yield numerous bioactive peptides with health benefits like antioxidant and antihypertensive effects. Bromelain enzyme shows the most promise for releasing diverse functional peptides from this sustainable plant protein source.
Area of Science:
- Food Science
- Biochemistry
- Proteomics
Background:
- Rapeseed meal, a byproduct of oil extraction, is a rich source of plant proteins.
- Bioactive peptides derived from plant proteins offer potential health benefits.
Purpose of the Study:
- To identify key proteins in cold-pressed rapeseed meal.
- To assess the potential of these proteins to release bioactive peptides using in silico hydrolysis.
- To evaluate the bioactivity of generated peptides.
Main Methods:
- Proteomic profiling using two-dimensional electrophoresis and MALDI-TOF/TOF mass spectrometry.
- In silico hydrolysis of identified protein sequences using BIOPEP database and plant proteases (papain, bromelain, ficin).
Main Results:
- Cruciferin was identified as the dominant protein in rapeseed meal.
- Over 50% of generated peptide fragments showed predicted bioactivities, including ACE and DPP-IV inhibition, antioxidant, neuroprotective, and anticancer effects.
- Bromelain yielded the highest quantity and diversity of bioactive peptides, with consistent identification of antihypertensive and antidiabetic peptides.
Conclusions:
- Rapeseed meal is a promising sustainable source of functional peptides.
- In silico hydrolysis effectively predicts the potential for generating bioactive peptides.
- Experimental validation is crucial to confirm the predicted bioactivities and therapeutic potential.
