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Updated: Sep 13, 2025
Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
Published on: March 13, 2017
CMOS-fabricated dielectrophoretic chip with embedded 3D TiN nano-electrode arrays for sperm capture and sperm damage
I-Hsuan Liao1, Jeng-Huei Shiau2, Chao-Min Cheng1
1Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Introduction:
This study developed an embedded 3D TiN nano-electrode arrays with a strong electric field and high biocompatibility for the effective capture of motile sperms. The chip integrates CMOS fabrication technology with a three-dimensional structural design, exhibiting both electrode-based dielectrophoresis and insulator-based dielectrophoresis characteristics. It provides a stable operating environment under strong electric fields with minimal Joule heating interference.
Methods:
In the experiments, we evaluated the effects of different waveforms and capture spaces on the capture efficiency of boar and bovine sperms.
Results And Discussion:
The results showed that square waves improved capture efficiency by approximately10% and confirmed that the capture space must exceed half the sperm length to enhance efficiency. When operated under a 20 Vpp square wave, the chip only generated a temperature rise of 1.7°C, causing no significant damage to the sperms and no notable decrease in viability. Under optimal conditions, the capture efficiencies for boar and bovine sperms reached 65.54% ± 1.07% and 63.25%, respectively. Overall, the results demonstrate that this chip offers high throughput, low Joule heating interference, and good species adaptability, showing potential for use in dynamic cell capture and high-throughput analysis.
