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Investigations of Cu2ONPs - Orange Peels: Biosynthesis, Characterization, and Anticancer Activity on a Cytotoxic
Khalidah Khalaf Jabbar1,2, Salim Oudah Mezan2, Mohamed Bouaziz1,3
1Laboratory of Electrochemistry and Environment, National School of Engineers of Sfax BP "1173" 3038, University of Sfax, Sfax, Tunisia.
Background:
The study aimed to evaluate the efficacy of nanocomposites extracted from orange peels in targeting and combating MCF-7 cells.
Methods:
We characterized the nanoparticles using XRD, FTIR, SEM, and GC-MS, and evaluated their cytotoxicity and activity using the MTT colorimetric assay and double staining technique.
Results:
The obtained results showed that the nanoparticles ranged from 19 to 66 nanometers in size and contained active compounds in the range of 4,000-500 cm⁻¹. However, the XRD sizes of the crystalline nanoparticles ranged from 15 to 23 nm. Orange peel contains important compounds such as octanal, 1, 6-octadien-3-ol, 3, 7-dimethyl, and limonene. At 800 mg/ml, DPPH can act as a strong antioxidant and reduce free radicals by 78.29%. The addition of the molecules resulted in a trace amount of 3.99%, a viability of 74.671%, and a half-maximal inhibitory concentration of 43.75 g/mL for the cell line. The compatibility with the low concentration effectively repaired the damaged cells.
Conclusions:
We expect the present study to provide important insights into the influence of molecular structure on anti-cancer action.
Insights
Orange peel nanocomposites show anti-cancer effects against MCF-7 cells, demonstrating potential for natural therapeutic development. These compounds effectively reduced cancer cell viability and repaired damaged cells, highlighting their therapeutic promise.
Area of Science:
- Nanotechnology
- Materials Science
- Biochemistry
Background:
- Orange peels contain valuable compounds with potential therapeutic applications.
- Nanocomposites derived from natural sources offer a promising avenue for drug development.
Purpose of the Study:
- To evaluate the efficacy of orange peel-derived nanocomposites against MCF-7 breast cancer cells.
- To characterize the physicochemical properties of these nanocomposites.
Main Methods:
- Nanoparticle characterization using XRD, FTIR, SEM, and GC-MS.
- Cytotoxicity and activity assessment via MTT assay and double staining.
- Evaluation of antioxidant activity using DPPH assay.
Main Results:
- Nanoparticles ranged from 19-66 nm, with crystalline sizes of 15-23 nm.
- Identified active compounds include limonene and octanal.
- Demonstrated significant antioxidant activity (78.29% radical reduction) and anti-cancer effects (IC50 of 43.75 µg/mL) on MCF-7 cells, with observed cell repair at low concentrations.
Conclusions:
- Orange peel nanocomposites exhibit potent anti-cancer properties.
- Molecular structure significantly influences anti-cancer action.
- These findings support the development of natural-based anti-cancer agents.
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