Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Discharge from Hospice Following Opioid Reduction.

Deutsches Arzteblatt international·2026

Same author

Inflammation reprograms fibro-adipogenic progenitors to sustain immunopathogenic niches in myositis.

Cell death & disease·2026

Same author

Software-defined Radar for MRI Motion Correction: A versatile, vendor-independent Platform.

medRxiv : the preprint server for health sciences·2026

Same author

Longitudinal Assessment of Muscle Involvement in Late-Onset Pompe Disease Using Quantitative MRI: A Prospective Cohort Study.

Journal of cachexia, sarcopenia and muscle·2026

Same author

Why pain biomarkers cannot replace the patient experience.

Nature neuroscience·2026

Same author

Case report: Parvovirus B19 encephalitis following rituximab therapy in a patient with immune-mediated thrombocytopenia.

Frontiers in immunology·2026

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies

Published on: January 3, 2011

Small Fiber Morphology and Function in Inclusion Body Myositis-A Multimodal Assessment Including Confocal Corneal

Dietrich Sturm^1,2, Anne-Katrin Güttsches^2,3, Matthias Vorgerd^2,3

¹Department of Neurology, Agaplesion Bethesda Krankenhaus, Wuppertal, Germany.

European Journal of Neurology

|July 29, 2025

Summary

Small nerve fiber pathology is common in inclusion body myositis (IBM), detected in most skin biopsies. Corneal confocal microscopy (CCM) showed lower sensitivity for diagnosing small fiber damage in IBM patients.

Keywords:

corneal confocal microscopy inclusion body myositis quantitative sensory testing skin biopsy small fiber neuropathy

More Related Videos

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

Published on: August 14, 2021

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Published on: December 8, 2021

Related Experiment Videos

Last Updated: Jun 18, 2026

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies

Published on: January 3, 2011

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

Published on: August 14, 2021

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Published on: December 8, 2021

Area of Science:

Neurology
Ophthalmology
Pathology

Background:

Inclusion body myositis (IBM) is a progressive neuromuscular disorder.
The extent of peripheral nerve involvement, particularly small nerve fibers, in IBM is not fully understood.
This study investigated small nerve fiber involvement in IBM using novel diagnostic techniques.

Purpose of the Study:

To evaluate small nerve fiber pathology in IBM patients.
To compare the diagnostic utility of skin biopsy, quantitative sensory testing, and corneal confocal microscopy (CCM) for small fiber assessment in IBM.
To explore the relationship between small fiber involvement and patient-reported pain and quality of life.

Main Methods:

Nineteen IBM patients underwent clinical and electrophysiological assessments.
Multimodal small fiber examination included skin biopsy, quantitative sensory testing, and CCM.
Standardized questionnaires assessed pain and quality of life.

Main Results:

Large fiber neuropathy was found in 67% of IBM patients.
Skin biopsy revealed small fiber pathology in 72% of cases, while CCM identified it in 32%.
Quantitative sensory testing primarily indicated large fiber damage (61%), with only 12% showing small fiber pathology.

Conclusions:

Small nerve fiber pathology is prevalent in IBM, particularly detectable via skin biopsy.
Corneal confocal microscopy (CCM) demonstrated lower sensitivity compared to skin biopsy for diagnosing small fiber pathology in IBM.
Further research is needed to optimize diagnostic strategies for small fiber involvement in IBM.