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Updated: Sep 13, 2025
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Rapid determination of amygdalin in almonds and almond-based products using lateral flow immunoassay based on gold
Xiaofang Li1, Lingling Guo1, Liqiang Liu1
1International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Abstract:
Amygdalin is a phytotoxin that releases hydrocyanic acid, a highly toxic substance, under the action of enzymes or acids, posing a threat to human health. In this study, a highly sensitive and specific monoclonal antibody (mAb) 3D11, with a half-maximal inhibitory concentration of 0.66 ng/mL, was developed through similarity calculation and molecular structure, molecular spatial overlap, electrostatic potential distribution, and HOMO-LUMO orbitals analyses of hapten design principles. A gold nanoparticle-based lateral flow immunoassay (GNP-LFIA) was developed to detect amygdalin in almonds and almond-based products, with a visual detection limit of 2-10 μg/kg and a detection limit of 0.27-1.27 μg/kg. The recoveries of GNP-LFIA ranged from 94.02 % to 109.50 %, and the accuracy and reliability were further confirmed by testing positive samples, demonstrating that GNP-LFIA can be a practical tool for the rapid on-site detection of amygdalin in almonds and almond-based products.
