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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

Updated: Sep 13, 2025

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Colorimetric ALP detection with a ligand-exchanged Au8 cluster.

Jingjing Yang1, Qing You2, Qian Wang1

  • 1Institutes of Physical Science and Information Technology, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Anhui University, Hefei, 230601, China. 20231@ahu.edu.cn.

Chemical Communications (Cambridge, England)
|July 30, 2025
PubMed
Summary

Atomically precise gold nanoclusters (Au8) show oxidase-like activity. This allows for sensitive colorimetric detection of alkaline phosphatase (ALP) by quenching superoxide radicals, demonstrating diagnostic potential.

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Last Updated: Sep 13, 2025

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Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
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Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

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Area of Science:

  • Nanomaterials Science
  • Biochemistry
  • Analytical Chemistry

Background:

  • Oxidase-like activity in nanomaterials is crucial for catalytic applications.
  • Superoxide radical (O2˙-) detection is important in biological and environmental monitoring.
  • Alkaline phosphatase (ALP) is a biomarker for various diseases.

Purpose of the Study:

  • To synthesize and characterize an atomically precise gold nanocluster with oxidase-like activity.
  • To investigate the potential of this nanocluster for the sensitive and specific detection of ALP.
  • To explore the diagnostic utility of the developed detection method.

Main Methods:

  • Synthesis of an atomically precise Au8 nanocluster using ligand exchange.
  • Characterization of the nanocluster's structure and properties.
  • Assay development for ALP detection based on superoxide radical quenching.

Main Results:

  • The synthesized Au8 nanocluster demonstrated significant oxidase-like catalytic activity.
  • ALP was found to effectively quench superoxide radicals in the presence of the Au8 nanocluster.
  • A sensitive and specific colorimetric method for ALP detection was established.

Conclusions:

  • Atomically precise Au8 nanoclusters possess inherent oxidase-like activity.
  • This activity enables a novel colorimetric approach for ALP detection.
  • The developed method shows promise for diagnostic applications.