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Updated: Sep 8, 2025

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Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons
Published on: February 10, 2016
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Imaging and Quantifying F-Actin Bundles in the Cortex of Mammalian Oocytes
Ghislain de Labbey1, Elvira Nikalayevich2
1Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Université PSL, Paris, France.
Methods in Molecular Biology (Clifton, N.J.)
|July 30, 2025
Abstract:
We describe the method of collection and microinjection of oocytes, acquisition of images of the oocyte cortex, as well as our image analysis pipeline to quantify the F-actin bundles in the oocyte cortex from these images. The metric is based on spatial correlations of the image intensity gradient.

