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Updated: Jun 23, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
First Human Infection Case of blaCTX-M-14 -Positive Klebsiella variicola in Japan
Yoshihiko Ogawa1, Yuki Suzuki2, Tatsuki Mizobe1
1Department of Infectious Diseases, Sakai City Medical Center, Japan.
Abstract:
Klebsiella variicola, often misidentified as Klebsiella pneumoniae, is gaining recognition as a pathogen. This study reports the first human case of infection with K. variicola harboringblaCTX-M-14 in Japan. MALDI time-of-flight mass spectrometry was used to confirm that clinical isolates from urine and blood cultures were K. variicola. The infection was successfully treated with cefmetazole. Whole-genome sequencing identified blaCTX-M-14 in an 8,066 bp plasmid. Given the rarity of extended-spectrum beta-lactamase-positive K. variicola infections in Japan, this case highlights the importance of accurately identifying and monitoring multidrug-resistant pathogens.
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