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Candidate imprinting control regions in dog genome
1Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.
BMC Genomics
|July 30, 2025
Summary
This study identifies potential genomic imprinting control regions (ICRs) in the Boxer dog breed using bioinformatics. These findings aid in understanding gene regulation and parent-specific gene expression in canines.
Area of Science:
- Genomics
- Epigenetics
- Mammalian Genetics
Background:
- Genomic imprinting regulates gene expression from parental alleles in mammals.
- Imprinting control regions (ICRs) govern this process but their identification in dogs remains challenging.
- Previous bioinformatics methods successfully located ICRs in other species.
Purpose of the Study:
- To identify candidate ICRs across the canine genome.
- To apply bioinformatics analyses for pinpointing novel ICR locations in the dog genome.
- To compare canine ICR candidates with known ICRs in other mammals.
Main Methods:
- Utilized bioinformatics analyses to identify CpG-rich motifs known as ZFBS-morph overlaps.
- Employed density plots to visualize cluster positions and identify peaks indicating candidate ICRs.
- Performed genome-wide analyses on the Canis familiaris (Boxer) breed.
Main Results:
- Successfully identified candidate ICRs within the Boxer dog genome.
- Several candidate ICRs were found in regions analogous to known ICR positions in mouse and human genomes.
- Specific candidate ICRs were identified for genes including MEST (PEG1), INPP5F_V2, PLAGL1 (ZAC1), IGF2R, PEG3, and GNAS.
Conclusions:
- The bioinformatics approach effectively pinpointed candidate ICRs in the canine genome.
- Identified candidate ICRs suggest conserved imprinting mechanisms across mammals.
- This research provides a foundation for further investigation into canine imprinting and its developmental roles.
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