Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.0K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.0K
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

562
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
562
Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

2.4K
Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
2.4K
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

969
An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
969
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

548
Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
548
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

1.1K
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Programmable Multi‑Modal Information Encryption System Based on the Dynamic Wetting Behavior of Liquid Metal.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Universal Rapid Machine Learning Models for Predicting Unconvoluted and Convoluted X-ray Absorption Spectra.

The journal of physical chemistry. A·2026
Same author

IPSBrain: A Unified Intelligent Data Analysis Platform for Multimodal Experimental Characterization at Advanced Photon Source.

The journal of physical chemistry. A·2026
Same author

Mechanisms of Ibuprofen Retention and Release in Dual-Responsive P(NIPAM-co-AAc) Nanogels: Coupling of Mesh Sieving and Affinity Switching.

Gels (Basel, Switzerland)·2026
Same author

Organosulfur compound-driven mobilization of colloidal metals and organic matter from acid mine drainage-contaminated paddy soils.

Journal of hazardous materials·2026
Same author

Structure-to-Therapeutic Transformation of Nanohydrogels Triggers Calcium Overload for Potentiated Boron Neutron Capture Therapy.

ACS nano·2026

Related Experiment Video

Updated: Sep 13, 2025

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy
06:00

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Published on: May 27, 2022

2.7K

XASDB: a new database of experimental interactive X-ray absorption spectra.

Xueqi Song1, Haodong Yao1, Fei Zhan1

  • 1Multi-Disciplinary Research Division, Institute of High Energy Physics, 19B Yuquan Road, Shijingshan District, Beijing 100049, People's Republic of China.

Journal of Synchrotron Radiation
|July 31, 2025
PubMed
Summary

We developed XASDB, a comprehensive database for X-ray absorption spectroscopy (XAS) data, offering visualization, processing, and matching tools. This platform enhances understanding and accessibility of XAS experimental data.

Keywords:
X-ray absorption spectroscopyXASMatchdatabase

More Related Videos

Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

9.2K
Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering
07:55

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Published on: April 17, 2018

12.8K

Related Experiment Videos

Last Updated: Sep 13, 2025

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy
06:00

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Published on: May 27, 2022

2.7K
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

9.2K
Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering
07:55

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Published on: April 17, 2018

12.8K

Area of Science:

  • Materials Science
  • Chemistry
  • Physics

Background:

  • Experimental X-ray absorption spectroscopy (XAS) generates complex datasets.
  • Existing platforms lack integrated tools for comprehensive XAS data management and analysis.
  • Efficiently accessing and analyzing XAS data is crucial for scientific advancement.

Purpose of the Study:

  • To introduce XASDB, a dedicated and integrated database for experimental XAS data.
  • To provide a user-friendly platform combining data visualization, processing, matching, and downloading.
  • To facilitate a holistic approach to understanding XAS data.

Main Methods:

  • Designed a MySQL database structure optimized for XAS data.
  • Developed professional plotting tools for data presentation.
  • Integrated data normalization, flexible searching, downloading options, and an XASMatch toolkit for spectral comparison.
  • Implemented an API for data sharing.

Main Results:

  • XASDB offers a unified solution for XAS data management.
  • The XASMatch toolkit enables identification and ranking of similar spectra.
  • The database currently contains 152 spectra of metals, oxides, and minerals standards.
  • An API facilitates interoperability with other data resources.

Conclusions:

  • XASDB provides a valuable resource for the XAS research community.
  • The platform streamlines the analysis and accessibility of experimental XAS data.
  • Future advancements, including high-energy photon sources, will expand the database's utility.