Related Experiment Video
Updated: Apr 11, 2026
Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
Published on: June 30, 2017
Protocol for the quantitative characterization of cell aggregates using an MRI setup maintaining optimal cultivation
Rebecca Wißmann1, Petros Martirosian2, Marina Danalache3
1Department of Diagnostic and Interventional Radiology, University Hospital of Tübingen, 72076 Tübingen, Germany.
None:
The use of destructive biochemical assays and the preparation of histologic samples are routinely employed to monitor development and viability of 3D cell aggregates. Magnetic resonance imaging (MRI) offers a non-destructive, high-resolution alternative to histological analysis, enabling longitudinal assessment of cellular dynamics while preserving sample integrity. Here, we present a protocol for non-invasive MR imaging of cell spheroid clusters by creating an adequate imaging environment. We describe steps for spheroid formation, casting of the imaging tube, cell cultivation, and data evaluation. For complete details on the use and execution of this protocol, please refer to Wißmann et al.1.
More Related Videos
10:25Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
Published on: March 9, 2021
10:13Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment
Published on: June 21, 2022
