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Humic Acid Regulates Root Growth through ROS-Dependent Pathway and Hormone Signaling in Rice
Andressa Fabiane Faria de Souza1, Andrés Calderín García2, José Nivaldo de Oliveira Sátiro1
1Plant Nutrition Laboratory, Department of Soils, Federal Rural University of Rio de Janeiro (UFRRJ), Seropédica 23890-000, Rio de Janeiro, Brazil.
Humic acid (HA) boosts rice root growth by influencing reactive oxygen species (ROS) and hormone pathways. This study clarifies how HA-mediated oxidative signals interact with hormones to promote plant development.
Area of Science:
- Plant Biology
- Biochemistry
- Agronomy
Background:
- Humic acid (HA) is known to enhance plant development.
- The precise mechanisms linking reactive oxygen species (ROS) and hormone signaling in HA-induced root growth are not fully understood.
Purpose of the Study:
- To investigate the role of vermicompost-extracted HA in rice root development.
- To elucidate the involvement of ROS and hormone signaling pathways in HA's effects on root growth.
Main Methods:
- Application of HA to rice plants.
- Utilized ROS scavengers and hormone pathway inhibitors.
- Performed gene expression analysis and ROS staining.
Main Results:
- HA significantly increased root dry weight (27%) and lateral root length (20%).
- Elevated O2•- and H2O2 levels were observed in specific root zones.
- Inhibitors of ROS and hormone pathways suppressed HA-induced growth.
- HA upregulated key genes involved in ROS and hormone signaling.
Conclusions:
- HA stimulates root development through a controlled oxidative signal that interacts with hormone pathways.
- This ROS-hormone crosstalk is a key mechanism of HA bioactivity.
- HA shows potential as a biostimulant for sustainable agriculture.
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