Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction Note: NSD2 targeting reverses plasticity and drug resistance in prostate cancer.

Nature·2026
Same author

Spatial remodeling of bone marrow architecture defines tissue-state signatures of disease activity and therapeutic response in myelodysplastic neoplasms.

Leukemia·2026
Same author

Reprogramming of TLR-Ferroptosis Signaling and Immunometabolic Pathways Overcomes Myeloid Suppression to Improve Checkpoint Blockade in Prostate Cancer.

Cancer research·2026
Same author

A unique transcriptomic landscape defines African-specific grade group 1 prostate cancer.

Research square·2026
Same author

Transcriptome sequencing of Hodgkin lymphoma Hodgkin and Reed-Sternberg cells reveals escape from NK cell recognition and an unfolded protein response.

Blood cancer journal·2026
Same author

Next-generation sequencing methodologies to identify patients for targeted therapy: focus on HR+/HER2- metastatic breast cancer.

Pathologica·2026
Same journal

Genome-wide SNP-based Profiling of Loss of Heterozygosity Reveals Distinct Molecular Subgroup-specific Patterns in Gastrointestinal Stromal Tumors (GIST).

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

AI-Assisted HER2 Scoring in Breast Cancer: Diagnostic Agreement and Understanding Discordance.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Corrigendum to "POU2F3 in Small Cell Lung Cancer (SCLC): Diagnostic Utility in Neuroendocrine-Low/Negative SCLC and Discrimination From Other Thoracic Malignancies and Other Small Blue Round Cell Tumors" [Laboratory Investigation 2026;106(6):106124].

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Assessing the Effects of a 3D Pathology Tissue-Processing Workflow on Downstream Molecular Analyses.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Transcription Factor Ets-1 is a Central Regulator of Redox Balance and Liver Regeneration Through EGF and TGF-β1 Signaling.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Analytical Sensitivity and HER2-Low: To Measure or Not to Measure, That Is the Question.

Laboratory investigation; a journal of technical methods and pathology·2026
See all related articles

Related Experiment Video

Updated: Sep 13, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.7K

Antibody-Based Multiplex Image Analysis: Standard Analytical Workflows and Artificial Intelligence Tools for

Mohamed Omar1, Giuseppe Nicolo' Fanelli2, Fabio Socciarelli3

  • 1Department of Computational Biomedicine, Cedars-Sinai Medical Center, Los Angeles, California; Cancer Therapeutics Program, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, California.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|July 31, 2025
PubMed
Summary
This summary is machine-generated.

Multiplex imaging in pathology allows simultaneous visualization of multiple biomarkers, offering a comprehensive view of tissue complexity. This review details digital image analysis workflows and open-source tools for multiplex IHC/IF, aiding pathologists in integrating these advanced techniques.

Keywords:
PathMLartificial intelligencecomputational pathologydigital pathologymultiplexed imagingspatial omics

More Related Videos

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

8.1K
Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors
06:32

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Published on: August 18, 2023

2.3K

Related Experiment Videos

Last Updated: Sep 13, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.7K
Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

8.1K
Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors
06:32

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Published on: August 18, 2023

2.3K

Area of Science:

  • Pathology
  • Digital Pathology
  • Biomedical Imaging

Background:

  • Conventional histopathology relies on visual inspection, while singleplex IHC is limited to single biomarkers.
  • Multiplexed imaging technologies (multiplex IHC/IF) enable simultaneous visualization of multiple biomarkers in a single tissue section.
  • These advanced methods provide quantitative multimarker data and spatial context, enhancing understanding of cellular interactions and disease mechanisms.

Purpose of the Study:

  • To review the standard digital image analysis workflow for multiplex imaging in pathology.
  • To discuss common open-source tools supporting each step of the analysis pipeline.
  • To provide practical guidance for pathologists and researchers integrating multiplex image analysis into routine workflows and translational research.

Main Methods:

  • Image acquisition and preprocessing
  • Cell segmentation techniques
  • Biomarker quantification strategies
  • Utilizing open-source software solutions

Main Results:

  • Detailed outline of an end-to-end digital image analysis pipeline for multiplex imaging.
  • Discussion of common challenges and solutions in processing large multichannel images.
  • Identification of open-source tools to support various stages of the workflow.

Conclusions:

  • Multiplex imaging offers a more comprehensive view of tissue pathology compared to conventional methods.
  • Standardized digital image analysis workflows and accessible open-source tools are crucial for adopting these technologies.
  • This review bridges the gap between advanced multiplex imaging and practical application in pathology and research.