Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

296
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
296
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

868
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
868
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

245
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
245
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

596
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
596
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

627
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
627
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

938
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
938

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D-Printed, Ultrastretchable Polychloroprene Elastomers via Thiol-ene Photopolymerization.

ACS applied materials & interfaces·2026
Same author

Multi-Isotope Internal Standardization for Inductively Coupled Plasma Mass Spectrometry.

ACS omega·2025
Same author

Leveraging Heterogeneous Catalyst Design Principles for Volatile PFAS Destruction through the Thermal Decomposition of CF<sub>4</sub>.

ACS omega·2025
Same author

Standard in the rinse.

Talanta·2025
Same author

Analysis of milk samples using calibration by proxy directly in the digestion vessel.

Talanta·2025
Same author

Multichannel Dilution Analysis Using a Single Peristaltic Pump Tube.

Applied spectroscopy·2025

Related Experiment Video

Updated: Sep 13, 2025

Emission Spectroscopic Boundary Layer Investigation during Ablative Material Testing in Plasmatron
09:41

Emission Spectroscopic Boundary Layer Investigation during Ablative Material Testing in Plasmatron

Published on: June 9, 2016

12.5K

Multi-wavelength internal standardization using inductively coupled plasma optical emission spectrometry.

Reagan Elia1, Thomas J Johnston1, Alberto D Figueroa1

  • 1Department of Chemistry and Biochemistry, University of North Florida, Jacksonville, FL, 32224, USA.

Analytica Chimica Acta
|August 1, 2025
PubMed
Summary

Multi-wavelength internal standardization (MWIS) offers a novel approach to trace analyte determination, improving accuracy and throughput over existing multi-signal methods. This technique enhances calibration strategies for complex samples using inductively coupled plasma optical emission spectrometry (ICP-OES).

Keywords:
CalibrationInductively coupled plasma optical emission spectrometry (ICP-OES)Internal standardsMatrix effectsMatrix-matchingMetals

More Related Videos

Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry
07:17

Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry

Published on: August 1, 2017

12.8K
A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS

Published on: July 11, 2017

10.9K

Related Experiment Videos

Last Updated: Sep 13, 2025

Emission Spectroscopic Boundary Layer Investigation during Ablative Material Testing in Plasmatron
09:41

Emission Spectroscopic Boundary Layer Investigation during Ablative Material Testing in Plasmatron

Published on: June 9, 2016

12.5K
Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry
07:17

Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry

Published on: August 1, 2017

12.8K
A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS

Published on: July 11, 2017

10.9K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Trace Analysis

Background:

  • Current trace analyte determination methods increasingly use "multi-signal" approaches, relying on instrumental signals for both calibration curve axes.
  • Existing multi-signal methods face limitations in sample throughput, matrix effect correction, and the requirement for multiple measurable signals per analyte.
  • Multi-wavelength internal standardization (MWIS) is introduced as a novel strategy to overcome these limitations by utilizing multiple emission wavelengths for analytes and internal standards.

Purpose of the Study:

  • To introduce and validate Multi-wavelength internal standardization (MWIS) as an advanced calibration technique.
  • To demonstrate MWIS's superiority over traditional and other multi-signal calibration methods.
  • To showcase MWIS's effectiveness in complex matrices and for analytes with limited spectral signals.

Main Methods:

  • Proof-of-concept established using inductively coupled plasma optical emission spectrometry (ICP-OES).
  • Spike recovery experiments conducted in complex matrices to assess accuracy and precision.
  • Comparison of MWIS performance against traditional calibration and other multi-signal techniques.
  • Validation using certified reference materials for comprehensive performance evaluation.

Main Results:

  • Analyte recoveries near 100% with relative standard deviations around 1% in complex matrices.
  • MWIS outperformed traditional calibration strategies and other multi-signal methods.
  • Certified reference material analysis yielded analyte recoveries between 90% and 118%.

Conclusions:

  • MWIS provides significant improvements over existing multi-signal calibration techniques, generating numerous calibration points from minimal solutions.
  • The method effectively handles analytes with few suitable emission wavelengths, such as Arsenic (As) and Lead (Pb), by monitoring multiple internal standard signals.
  • Spectral interferences are readily identifiable and can be excluded from calculations, simplifying data analysis.