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Updated: Sep 13, 2025

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
Tilapia IFP35 is differently induced by three subgroups of type I IFNs via the JAK-STAT signaling
Lihua Li1, Jiaqiao Cai2, Zihao Wu2
1Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, and Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institute, College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, and Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, China.
Abstract:
Interferons (IFNs) trigger effective antiviral responses by activating the expression of a series of interferon-stimulated genes (ISGs), and interferon-induced protein 35 (IFP35) has been identified as a typical ISG in mammals and fish. Nevertheless, it is required to compare distinct responses of IFP35 to different subgroups of type I IFNs in fish. Here, we cloned IFP35 gene (named as On-IFP35) from tilapia, and syntenic analysis showed that On-IFP35 gene was found in a locus conserved in cartilaginous and bony fishes. Expression analyses showed that On-IFP35 was detectable in all organs/tissues tested except for spleen, and was highly induced after poly (I:C) treatment in vivo and in vitro. In addition, the expression of On-IFP35 was differently induced by three subgroups of type I IFNs, and these induction effect depended on the JAK-STAT signaling. Consistently, luciferase activity analysis revealed that the promoter of On-IFP35 was differently activated by three subgroups of type I IFNs via a ISRE site. The present results thus contribute to better understanding of the transcriptional regulation of fish IFP35 in type I IFN response.
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