A simplified protocol for vitrification of hamster embryos.

Area of Science:

• Reproductive Biology
• Cryobiology
• Animal Models

Background:

• Golden hamsters (Mesocricetus auratus) are valuable biomedical research models.
• Genome-editing technology enables the creation of unique hamster models not possible in mice or rats.
• Efficient cryopreservation of hamster embryos is crucial for advancing research using these models.

Purpose of the Study:

• To develop and present a simplified vitrification protocol for golden hamster embryo preservation.
• To establish a reproducible method for cryopreserving hamster embryos for research purposes.

Main Methods:

• In vivo-derived 8-cell or morula hamster embryos (Day 3) were vitrified using the Cryotop method.
• A modified HECM-3 medium containing ethylene glycol, DMSO, and sucrose was utilized for vitrification.
• Warmed embryos were transferred to the uteri of Day 3-pregnant foster females.

Main Results:

• The simplified vitrification protocol resulted in 21-26% of transferred embryos developing to term.
• The protocol was successfully implemented in a standard laboratory setting without specialized equipment.
• The method demonstrated reproducibility, indicating broad applicability.

Conclusions:

• A simplified and reproducible vitrification protocol for golden hamster embryos has been established.
• This technique supports the use of gene-edited hamster models in biomedical research.
• The protocol's applicability extends to laboratories utilizing hamsters for research.