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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
Published on: August 25, 2018
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Splice, exchange, extend: precision editing of native proteins in live cells
Kevin Schiefelbein1, Yael David2
1Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Trends in Biotechnology
|August 3, 2025
Abstract
None:
Beyer et al. and Hua et al. developed platforms for precise chemical editing of proteins in living mammalian cells. These approaches enabled site-specific tagging at diverse protein sites without disrupting function. Demonstrated on several key proteins, this technology opens new possibilities for studying protein dynamics and functions in live cells.
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