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Related Concept Videos

Microbiome of the Eye01:22

Microbiome of the Eye

67
The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...
67

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Investigating Microinvasive Intra-Ocular Biopsy.

Jared Ching1,2, Shohei Kitahata1, Hinako Ichikawa1

  • 1Department of Ophthalmology and Microtechnology, Yokohama City University Medical Centre, Yokohama, Japan.

Clinical & Experimental Ophthalmology
|August 4, 2025
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Summary

Microneedles show promise for minimally invasive intraocular biopsy, successfully aspirating ocular cancer cells with high viability. These microneedles create self-sealing wounds, reducing leakage in anterior and posterior segments.

Keywords:
intra‐ocular biopsymicroneedlesmicro‐invasive biopsyretinoblastomauveal melanoma

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Area of Science:

  • Ophthalmology
  • Biomedical Engineering
  • Surgical Innovation

Background:

  • Current intraocular biopsy methods using small gauge needles and subretinal cannulae risk wound leakage.
  • Investigating microneedles with internal diameters as small as 49G for improved intraocular biopsy.
  • Focus on both posterior and anterior segments of the eye.

Purpose of the Study:

  • To evaluate the feasibility of using microneedles for intraocular biopsy.
  • To assess cell viability after aspiration with microneedles.
  • To determine the wound sealing properties of microneedle punctures in ocular tissues.

Main Methods:

  • Aspiration of human uveal melanoma and retinoblastoma cell lines using various microneedle sizes.
  • In vitro analysis of cell viability post-aspiration.
  • Ex vivo porcine eye model for simulating retinal puncture and anterior chamber puncture with microneedles.
  • Optical coherence tomography (OCT) and histology for imaging and analysis.

Main Results:

  • Microneedles successfully aspirated ocular cancer cells (retinoblastoma, uveal melanoma) with high cell viability (72.83% for 49G).
  • A 49G microneedle created a self-sealing retinal wound in ex vivo porcine eyes, preventing reflux of 200nm microbeads.
  • Anterior chamber puncture with microneedles resulted in 0% aqueous leakage, significantly outperforming larger gauge needles.

Conclusions:

  • A microinvasive approach using microneedles for intraocular biopsy is feasible.
  • Microneedles offer a promising alternative to current methods, minimizing leakage.
  • Further in vivo studies are warranted to validate these findings.