Related Experiment Video
Updated: Sep 12, 2025
08:14
Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
Published on: October 28, 2014
12.9K
Induced Pluripotent Stem Cells derived CD71＋CD235a＋ Erythroblasts Were Increased by Sirtuin 1 Activator
Changyeong Kim1,2, Kyung Hwan Park1,2, Soo-Been Jeon3
1Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea.
International Journal of Stem Cells
|August 4, 2025
Summary
Sirtuin 1 activation enhances the proliferation of erythroblasts derived from induced pluripotent stem cells (iPSCs). This finding supports Sirtuin 1
Area of Science:
- Stem cell biology
- Regenerative medicine
- Hematopoiesis
Background:
- Induced pluripotent stem cells (iPSCs) are a valuable source for regenerative medicine.
- Large-scale production of functional red blood cells (RBCs) requires efficient erythroblast proliferation.
- Small molecules can optimize iPSC differentiation, but Sirtuin 1's role in erythroblast proliferation is unknown.
Purpose of the Study:
- To investigate the specific effects of Sirtuin 1 on erythroblast proliferation from iPSCs.
- To determine if Sirtuin 1 activation can enhance the generation of erythroblasts post-endothelial-to-hematopoietic transition (EHT).
Main Methods:
- Developed a protocol to study Sirtuin 1 effects on erythroblasts after EHT.
- Analyzed erythroblast populations using specific cell surface markers (CD71 and CD235a).
Main Results:
- Sirtuin 1 activation significantly increased the frequency of CD71+CD235a+ erythroblasts.
- This effect was observed at the early stage following EHT, indicating a role in proliferation.
Conclusions:
- Sirtuin 1 activation promotes erythroblast proliferation from iPSCs.
- Sirtuin 1 activation is a potential strategy for improving large-scale RBC cultures for clinical applications.
Related Concept Videos
01:22iPS Cell Differentiation
2.8K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.8K
01:29Somatic to iPS Cell Reprogramming
2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K
01:06Induced Pluripotent Stem Cells
4.4K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.4K