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Updated: Sep 12, 2025

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation
Published on: April 27, 2021
Cashew nut oligopeptide inhibits mast cell degranulation by suppressing activation of the NF-Κb signalling pathway
Di Chen1,2, Xin Zhou1, Yan Zhang3
1Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Engineering Research Center of Utilization of Tropical polysaccharide resources, Ministry of Education; College of Food Science and engineering, Hainan University, Haikou 570228, PR China.
Abstract:
This study aimed to isolate oligopeptides with anti-allergic properties from cashew nuts. Cashew nut protein was extracted using subcritical water extraction. Subsequently, cashew nut oligopeptides (COPs) were obtained via a two-step enzymatic hydrolysis involving alkaline proteinase and bromelain. Mass spectrometry analysis identified a specific COP with the sequence Ile-Ile-Ala-Ile-Pro-Ala-Gly-Val-Ala-His. This COP was synthesised to investigate its impact on preventing degranulation. Analysis revealed that COP effectively inhibited the release and production of proinflammatory mediators, including tumour necrosis factor-α, interleukin-6, histamine and β-hexosaminidase and influx of extracellular calcium ions. Furthermore, COP significantly suppressed degranulation and inflammatory responses by inhibiting activation of nuclear factor kappa B signalling pathway in allergic mast cells. These findings suggest that COP possesses strong anti-allergic effects by specifically targeting mast cells degranulation. This s highlights COP's therapeutic potential against allergic inflammatory disorders, and its promising role in treating allergic diseases.
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