High-Selectivity Nonenzymatic Creatinine Sensor Using Electrografted Ionic Liquid and Nafion for Reliable Clinical...

Area of Science:

• Electrochemistry
• Materials Science
• Biomedical Engineering

Background:

• Accurate creatinine detection is crucial for kidney function monitoring and diagnosing renal impairments.
• Nonenzymatic catalysts offer improved sensitivity but often lack selectivity, limiting practical applications.
• Existing methods face challenges with interference and stability in real-world samples.

Purpose of the Study:

• To develop a novel nonenzymatic creatinine sensor with enhanced selectivity and stability.
• To utilize copper(II) oxide catalyst with styrenyl-triphenylphosphonium-based ionic liquid (STPP-IL) and Nafion for improved performance.
• To validate the sensor's capability for selective creatinine quantification in biological samples.

Main Methods:

• Fabrication of a modified electrode using electrografting for uniform STPP-IL coverage.
• Characterization of the electrode surface using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS).
• Electrochemical detection of creatinine within a specific concentration range in the presence of interferents.

Main Results:

• The modified sensor exhibited a wide detection range (1.5–800 μM) with high sensitivity and low standard error.
• Selective quantification of creatinine in artificial and natural human sweat was achieved, demonstrating minimal interference.
• The sensor maintained over 90% of its initial response after 40 days of storage and showed high reproducibility (1.3% relative error).

Conclusions:

• The developed nonenzymatic creatinine sensor demonstrates superior selectivity, stability, and reproducibility.
• The sensor shows significant potential for reliable creatinine detection in point-of-care diagnostic applications.
• This advancement offers a promising alternative for noninvasive kidney function monitoring.