Related Experiment Video

Updated: Sep 12, 2025

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

7.6K

Dysregulation of MicroRNA-455-5p Contributes to Pulpitis Pathogenesis Through Modulating MYD88

Qidi Hu1, Yihan Fu2, Hao Xu3

  • 1Department of Stomatology, Yuyao Maternity and Child Health Care Hospital, Ningbo, China.

International Dental Journal
|August 5, 2025
PubMed
Abstract

Insights

MicroRNA-455-5p (miR-455-5p) is significantly reduced in pulpitis, impacting cell activity, inflammation, and oxidative stress. Restoring miR-455-5p levels offers a potential therapeutic strategy for pulpitis.

Area of Science:

  • Molecular Biology
  • Dental Research
  • Biochemistry

Background:

  • Pulpitis involves complex cellular and molecular changes.
  • MicroRNAs play crucial roles in regulating gene expression and cellular processes.
  • Understanding the specific microRNAs involved in pulpitis is essential for developing targeted therapies.

Purpose of the Study:

  • To investigate the role of microRNA-455-5p (miR-455-5p) in the initiation and progression of pulpitis.
  • To explore the diagnostic potential of miR-455-5p in pulpitis.
  • To elucidate the molecular mechanisms underlying miR-455-5p's function in dental pulp cells.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) for miR-455-5p expression analysis.
  • Receiver operating characteristic (ROC) analysis to assess diagnostic capacity.
  • In vitro studies on human dental pulp cells (hDPCs) to evaluate impacts on cellular activity, inflammation, and oxidative stress.
  • Bioinformatic analysis, luciferase reporter assays, and Pearson's correlation to identify and confirm miR-455-5p targets.

Main Results:

  • miR-455-5p was significantly downregulated in pulpitis patients and LPS-induced hDPCs, showing high diagnostic potential.
  • Enforced miR-455-5p expression improved cell viability, reduced apoptosis, and alleviated inflammation and oxidative stress in a pulpitis model.
  • MYD88 was identified as a direct target of miR-455-5p, with a negative correlation observed. MYD88 overexpression reversed the protective effects of miR-455-5p.

Conclusions:

  • Dysregulated miR-455-5p is implicated in pulpitis development and progression by affecting cellular behaviors, inflammation, and oxidative stress.
  • miR-455-5p serves as a potential diagnostic biomarker for pulpitis.
  • Modulating miR-455-5p offers a novel therapeutic avenue for managing pulpitis.
Keywords:
Cell viabilityInflammatoryMYD88MicroRNA-455-5pPulpitis

More Related Videos

Inducing Apical Periodontitis in Mice10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

12.3K
Author Spotlight: Enhancing Dental Pulp Research with Improved Mouse Models05:16

Author Spotlight: Enhancing Dental Pulp Research with Improved Mouse Models

Published on: October 27, 2023

1.2K

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.4K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.2K
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
7.3K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.9K