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Development and characterization of the genotype F attenuated mumps candidate strains

Hongtao Liu1,2,3, Na Liu1, Yueqiu Sun1

  • 1Vaccines R&D Department, Changchun Keygen Biological Products Co., Ltd., Changchun, Jilin, China.

Frontiers in Immunology
|August 6, 2025
PubMed
Abstract

Insights

Two new mumps virus (MuV) genotype F vaccine candidates were developed. These strains show strong immunogenicity and low neurotoxicity, addressing concerns about current vaccine effectiveness against circulating MuV strains.

Area of Science:

  • Virology
  • Vaccinology
  • Immunology

Background:

  • Mumps virus (MuV) causes acute infections, potentially affecting the nervous system.
  • The prevalent MuV genotype F in China differs from genotype A vaccine strains, impacting immunization effectiveness.

Purpose of the Study:

  • To develop and evaluate novel mumps virus genotype F candidate vaccines.
  • To assess the immunogenicity and safety of these new strains.

Main Methods:

  • Isolation of a genotype F MuV strain from patients.
  • Preparation of two candidate vaccine strains (QBB-2BS-3.2 and QBB-2BS-9.3) through cell adaptation and plaque purification.
  • Evaluation of immunogenicity (neutralizing antibodies, cell-mediated immunity) in mice and neurotoxicity in rats.

Main Results:

  • Both candidate strains induced significant neutralizing antibody and cell-mediated immune responses in mice.
  • Neurotoxicity assessments showed minimal adverse effects, comparable to the existing S79 vaccine strain.

Conclusions:

  • Two attenuated genotype F MuV candidate strains were successfully created.
  • These strains demonstrate promising immunogenicity and safety, supporting the development of genotype F mumps live attenuated vaccines.
Keywords:
F genotypeQBB strainimmunogenicitymumps virusneurovirulence

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