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The Lipid Interactome: An interactive and open access platform for exploring cellular lipid-protein interactomes

Gaelen Guzman¹, André Nadler², Frank Stein³

¹= Departments of Molecular Immunology and Immunology and Chemical Physiology and Biochemistry, Oregon Health & Science University, Portland, OR, USA.

|August 6, 2025

Summary

The Lipid Interactome database offers a centralized resource for exploring protein-lipid interactions. It harmonizes proteomics data, enabling researchers to better understand lipid functions in cellular signaling and membrane dynamics.

Area of Science:

Biochemistry
Proteomics
Cell Biology

Background:

Lipid-protein interactions are crucial for cellular signaling and membrane dynamics.
Systematic characterization of these interactions is challenging due to lipid biochemical properties.
Recent advances in functionalized lipid probes have improved proteomics-based identification of interacting proteins.

Purpose of the Study:

To address the lack of a centralized resource for lipid interactome data.
To create a structured, interactive web portal adhering to FAIR data principles.
To enable systematic exploration and comparison of lipid interactome studies.

Main Methods:

Development of a web portal for lipid interactome data.
Standardized data formatting for interoperability.
Implementation of interactive visualizations and cross-study comparison tools.

Main Results:

Consolidation and curation of lipid interactome proteomics data from multiple studies.
Creation of a Findable, Accessible, Interoperable, and Reusable (FAIR) data resource.
Enabling researchers to explore protein partners of bioactive lipids.

Conclusions:

The Lipid Interactome database serves as a critical tool for deciphering lipid functions.
It facilitates a deeper understanding of lipid roles in cellular systems.
The resource promotes data integration and reuse in lipidomics research.

Keywords:

Lipid-protein interactions database interactomics multifunctionalized lipids photoaffinity labeling proteomics

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