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Nonthermal Atmospheric Plasma Induces Osteogenic Activity in Osteoblasts through the ROS/WNT Signaling Axis.

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Nonthermal plasma (NTP) treatment of media stimulates osteoblast activity by enhancing bone formation markers and collagen production. This process involves reactive oxygen species (ROS) and the WNT signaling pathway, offering a potential therapeutic strategy for bone regeneration.

Keywords:
WNT signaling pathwaynonthermal plasmaosteoblastsosteogenic activityreactive oxygen species

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Area of Science:

  • Biomedical Engineering
  • Plasma Physics
  • Cell Biology

Background:

  • Nonthermal plasma (NTP) shows promise for biological applications.
  • NTP's influence on osteoblast activity and its mechanisms remain unclear.
  • Investigating NTP's effect on osteogenic activity is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the osteogenic potential of NTP-treated media on osteoblasts.
  • To elucidate the underlying mechanisms, including signaling pathways and reactive oxygen species (ROS).
  • To explore NTP as a therapeutic strategy for bone formation.

Main Methods:

  • Treatment of cell culture media with dielectric barrier discharge (DBD) and jet NTP (JNTP).
  • Indirect exposure of osteoblasts to NTP-treated media (JNTPM).
  • Analysis of ALP activity, osteogenic gene expression, collagen production, mineralization, WNT pathway activation, and ROS levels.

Main Results:

  • JNTP-treated media (JNTPM) significantly induced ALP activity and osteogenic marker expression in osteoblasts.
  • JNTPM enhanced collagen production, mineralization, and WNT signaling pathway activation (β-catenin stabilization, GSK3-β phosphorylation).
  • Intracellular ROS levels increased with JNTPM treatment, and ROS inhibition reduced WNT signaling and osteogenic activity.

Conclusions:

  • JNTPM treatment stimulates osteogenic activity in osteoblasts.
  • The osteogenic effects are mediated by the cross-talk between ROS and the WNT signaling pathway.
  • NTP-treated media presents a potential therapeutic approach for enhancing bone formation.