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Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

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Published on: November 2, 2012

Advances in metal-DOTA/DTPA complexes for mass cytometry.

Yin-Feng Wang¹, Wenying Wu¹, Guojun Han¹

¹Institute of Advanced Clinical Medicine, Biomedical Engineering Department, International Cancer Institute, Peking University, Beijing 100083, China. wangyf10@bjmu.edu.cn.

|August 7, 2025

Summary

Mass cytometry uses stable isotopes instead of fluorophores for high-resolution single-cell analysis. Metal-DOTA/DTPA complexes are key reagents, offering stability and versatile conjugation for advanced applications.

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Area of Science:

Biotechnology
Analytical Chemistry
Immunology

Background:

Mass cytometry offers high-resolution, high-throughput single-cell analysis.
It overcomes spectral limitations of flow cytometry using stable isotope tags.
Metal-chelating complexes like DOTA/DTPA are crucial mass tag reagents.

Purpose of the Study:

To review mass tag reagent mechanisms and metal-DOTA/DTPA complex design.
To classify DOTA/DTPA-based reagents.
To explore their applications in mass cytometry.

Main Methods:

Review of mass tag reagent working principles.
Analysis of metal-DOTA/DTPA complex design and properties.
Classification of DOTA/DTPA-based reagents (polymers, microbeads, small molecules).

Main Results:

DOTA/DTPA chelators provide thermodynamic stability and kinetic inertness.
These chelators exhibit versatile conjugation chemistry for reagent development.
Diverse DOTA/DTPA-based reagents are suitable for mass cytometry.

Conclusions:

Metal-DOTA/DTPA complexes are essential for advanced mass cytometry.
Their stability and conjugation versatility enable robust single-cell analysis.
This review provides a comprehensive overview of these reagents and their applications.