Related Experiment Video
Updated: Sep 12, 2025
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
Published on: July 16, 2020
Advances in native cell membrane nanoparticles system
1Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA 23298-0540, USA; Center for Drug Discovery, Virginia Commonwealth University, Richmond, VA 23298-0133, USA.
Abstract:
The native cell membrane nanoparticles (NCMN) system utilizes membrane-active polymers specifically designed and optimized to extract and stabilize membrane proteins in the form of NCMN particlesfor biochemical and biophysical characterization. The NCMN system is a genuine and advanced detergent-free approach inspired by the membrane activity of the styrene-maleic acid copolymers (SMA), distinguishing it from the nanodisc technology, Salipro technology, and Peptidisc technology. This review introduces the current advancements in the NCMN system, including the development of NCMN polymers, the application of the NCMN system for single-particle cryo-EM analysis, and the functional characterization of membrane proteins.
