Related Experiment Video
Updated: Sep 12, 2025
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
Published on: August 23, 2024
Photoelectrochemical biosensor based on Bi2S3/Bi2O2CO3 for lactic acid detection
Zhongzhou Si1,2, Rong Shao3, Ting Li4,5
1Department of Liver Transplantation, The Second Xiang-Ya Hospital, Central South University, Changsha, 410011, China.
None:
Photoelectrochemical biosensor based on Bi2S3/Bi2O2CO3 heterojunction as photoelectrically active material and lactate oxidase was constructed for sensitive detection of lactic acid in serum. Topological transformation method was utilized to prepare Bi2S3/Bi2O2CO3 composites, which can generate electron-hole pairs triggering photoelectrochemical reactions under light illumination. The Bi2S3/Bi2O2CO3 heterojunction effectively promoted the separation and transportation of photogenerated electrons and holes, leading to improved photoelectric conversion efficiency. Lactic acid is catalyzed by lactate oxidase to generate H2O2, which reacts with photogenerated holes, leading to photocurrent increase, achieving quantitative detection of lactic acid. The prepared biosensor has a wide detection range for lactic acid from 0.01 mM to 10 mM, with detection limit as low as 0.006 mM. In addition, the detection of lactic acid in serum samples further validated the potential application of the sensor.
