Related Experiment Video

Updated: Sep 12, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
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Exploring Hub Gene-Mediated Signaling and miRNA Interactions in Wilms Tumor Development and Drug Sensitivity

Huajun Yang1, Yunxiao Zhang2, Xianming Yao1

  • 1Department of Urology, Hangzhou Children's Hospital, Hangzhou, Zhejiang Province, 310000, China.

Current Pharmaceutical Biotechnology
|August 8, 2025
PubMed
Abstract

Insights

Key genes SLC12A3, ADH6, GSTM3, and CLCNKB are dysregulated in Wilms tumor (WT), showing potential as diagnostic biomarkers and therapeutic targets for this pediatric kidney cancer.

Area of Science:

  • Oncology
  • Genomics
  • Molecular Biology

Background:

  • Wilms tumor (WT) is a common pediatric kidney cancer with complex molecular drivers.
  • Current treatments offer suboptimal prognosis for high-risk cases, necessitating new diagnostic and therapeutic strategies.

Purpose of the Study:

  • To identify key molecular mechanisms in WT by pinpointing hub genes.
  • To evaluate the diagnostic and prognostic potential of identified hub genes in WT.

Main Methods:

  • Differential gene expression analysis using Gene Expression Omnibus (GEO) datasets.
  • Protein-protein interaction (PPI) network analysis to identify hub genes.
  • Functional assays (proliferation, colony formation, wound healing) and prognostic value assessment.

Main Results:

  • SLC12A3, ADH6, GSTM3, and CLCNKB identified as significantly dysregulated hub genes in WT.
  • These genes demonstrate high diagnostic potential (sensitivity, specificity) and prognostic significance for WT patient survival.
  • Functional studies indicated SLC12A3 and ADH6 overexpression inhibit tumor progression.

Conclusions:

  • SLC12A3, ADH6, GSTM3, and CLCNKB play critical roles in WT pathogenesis.
  • These genes represent promising diagnostic biomarkers and potential therapeutic targets for Wilms tumor management.
Keywords:
Wilms tumorbiomarkerhub genepathogenesistreatment.

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