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Updated: Sep 12, 2025
07:14
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
Published on: September 20, 2019
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Dynamin regulates PLK-1 localization and spindle pole assembly during mitosis.
Carter Dierlam1, Livinus Anyanwu2, Stephanie Held3
1Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, MD 20742.
Biorxiv : the Preprint Server for Biology
|August 8, 2025
Summary
The dynamin homolog DYN-1 regulates mitotic spindle poles and Polo-like kinase 1 (PLK-1) localization. Depleting DYN-1 disrupts PLK-1 removal from centrosomes, causing cytokinesis defects.
Area of Science:
- Cell Biology
- Molecular Biology
- Genomics
Background:
- Accurate cytokinesis is crucial for genomic stability.
- The GTPase dynamin's role in mitosis is largely unknown, despite its established function in vesicular transport.
Purpose of the Study:
- To investigate the function of the C. elegans dynamin homolog, DYN-1, during mitosis.
- To determine DYN-1's role in mitotic spindle pole assembly and Polo-like kinase 1 (PLK-1) localization.
Main Methods:
- Depletion of DYN-1 in C. elegans embryos.
- Analysis of mitotic spindle pole morphology.
- Assessment of PLK-1 localization at centrosomes and midbody.
- Depletion of DNM2 (human DYN-1 homolog) in HeLa cells.
Main Results:
- DYN-1 depletion caused enlarged metaphase spindle poles and increased centrosome-associated PLK-1.
- PLK-1 failed to relocate from centrosomes to the midbody in DYN-1-depleted embryos, leading to midbody defects.
- Total PLK-1 protein levels were not altered by DNM2 depletion in HeLa cells.
Conclusions:
- DYN-1 is a novel regulator of PLK-1 localization during mitosis.
- Impaired PLK-1 removal from centrosomes upon DYN-1 depletion may cause cytokinesis defects.
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