Aromatic and nitrogen-containing acid separation by reversed-phase high performance liquid chromatography and...

Area of Science:

• Analytical Chemistry
• Biochemistry
• Chromatography

Background:

• Accurate separation and identification of organic acids are crucial in various scientific fields.
• Existing methods may face challenges in resolving complex mixtures of aromatic and nitrogen-containing acids.

Purpose of the Study:

• To develop and present a new method for the separation and identification of aromatic and nitrogen-containing acids.
• To demonstrate the utility of this method in analyzing biological samples.

Main Methods:

• Reversed-phase high-performance liquid chromatography (HPLC) was employed for acid separation.
• Detection and identification were achieved using ultraviolet (UV) spectra obtained from a photodiode array detector (DAD).
• Peak height ratios and complete UV spectra were utilized for characterization.

Main Results:

• Successfully separated and identified 57 different acid standards.
• Demonstrated the capability of UV spectra from DAD for acid identification.
• Reported peak height ratios and displayed complete UV spectra for the analyzed acids.

Conclusions:

• The developed HPLC-UV/DAD method provides effective separation and identification of aromatic and nitrogen-containing acids.
• The technique is applicable to the analysis of complex biological matrices, such as human urine.
• This method offers a valuable tool for metabolic profiling and clinical diagnostics.