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Updated: Jun 23, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
High diagnostic accuracy of pooled samples analysed with a commercial PCR for detecting MRSA carriage
Ditte Bang Oturai1, Jonas Bredtoft Boel2, Jette Bangsborg1
1Department of Clinical Microbiology, Copenhagen University Hospital - Herlev and Gentofte, Herlev, Denmark.
Abstract:
We evaluated the diagnostic accuracy of two Cepheid Xpert MRSA assay generations using pooled samples from 4,519 patients to detect MRSA carriage in a low-prevalence population. High accuracy, including 95 % sensitivity and 99 % specificity was shown for the Xpert MRSA NxG kit. This supports pooled PCR as a resource-efficient method.
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