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Updated: May 14, 2026
Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits
Published on: March 16, 2016
Protocol for a two-clone system to study signaling interactions among neuronal cells in a pre-clinical Alzheimer's
Aditi Singh1, Prajakta Deshpande1, Ankita Sarkar1
1Department of Biology, University of Dayton, Dayton, OH 45469, USA.
Abstract:
This protocol outlines a two-clone genetic mosaic approach in Drosophila to study cell signaling using the FLP/FRT (Flippase/Flippase recombination target) and Gal4/UAS (upstream activating sequence)/Gal80 systems. We generated a stable transgenic line expressing human Aβ42, and then we crossed it with a GFP-marked FRT line to produce mosaic clones via heat-shock-induced recombination. Then we dissected, stained, and imaged the eye discs to visualize wild-type and mutant clones. Image analysis with Fiji/ImageJ and statistical quantification in GraphPad Prism enable the study of cell competition and intercellular signaling mechanism(s) in human diseases. For complete details on the use and execution of this protocol, please refer to Yeates et al. and Tare et al.1,2.
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