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Related Experiment Video

Updated: Sep 11, 2025

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Published on: June 5, 2020

Advancing Genotoxicity Assessment by Building a Global AOP Network.

E Demuynck^1,2, T Vanhaecke², A Thienpont¹

¹Scientific Direction of Chemical and Physical Health Risks, Sciensano, Brussels, Belgium.

Environmental and Molecular Mutagenesis

|August 11, 2025

Summary

This summary is machine-generated.

Modernizing genotoxicity testing requires Integrated Approaches for Testing and Assessment (IATAs). These frameworks use New Approach Methodologies (NAMs) to improve accuracy and reduce animal use.

Keywords:

AOP IATA NAMs genotoxicity

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Area of Science:

Toxicology
Genetics
Regulatory Science

Background:

Current genotoxicity testing faces challenges like misleading results and limited mechanistic insights.
Existing testing batteries lack integration of advanced technologies and quantitative assessment.
There's a need to reduce reliance on animal testing in genotoxicity assessment.

Purpose of the Study:

To explore challenges in European regulatory frameworks for genotoxicity assessment.
To discuss the development and implementation of Integrated Approaches for Testing and Assessment (IATAs) for genotoxicity.
To present a global Adverse Outcome Pathway (AOP) network for DNA damage to guide IATA development.

Main Methods:

Review of current genotoxicity testing strategies and regulatory frameworks.
Exploration of New Approach Methodologies (NAMs) like 3D systems, ToxTracker, and transcriptomic biomarkers.
Application of the Adverse Outcome Pathway (AOP) framework to integrate NAMs into IATAs.

Main Results:

Identified key challenges and gaps in current genotoxicity assessment, particularly within European regulations.
Highlighted the potential of NAMs and AOPs to create more accurate and efficient genotoxicity testing.
Proposed a global AOP network for permanent DNA damage to support regulatory decision-making.

Conclusions:

IATAs integrating NAMs, guided by AOPs, are essential for modernizing genotoxicity assessment.
This approach can enhance accuracy, efficiency, and ethical standards while reducing animal testing.
The proposed AOP network offers a framework for improved regulatory decision-making in chemical safety assessment.