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Related Experiment Video

Updated: Sep 11, 2025

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections

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Published on: February 2, 2024

Haemophilus influenzae Acute Prosthetic Joint Infection: A Rare Case Report on Time-Critical, Pathogen-Specific

Shreya Chaudhuri¹, Rahul Kakran², Vipin Tyagi²

¹Department of Clinical Microbiology and Infectious Diseases, Yashoda Group of Hospitals, Greater Noida, Uttar Pradesh, India.

Journal of Orthopaedic Case Reports

|August 11, 2025

Summary

Rapid syndromic multiplex PCR accurately identified Haemophilus influenzae in a prosthetic joint infection (PJI). This enabled prompt debridement, antibiotic therapy, and implant retention, improving patient outcomes.

Keywords:

Haemophilus influenzae Prosthetic joint infection culture-negative infection debridement and implant retention rapid pathogen identification syndromic diagnosis

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Area of Science:

Orthopedics
Infectious Diseases
Molecular Diagnostics

Background:

Prosthetic joint infections (PJIs) are severe complications of arthroplasty.
Haemophilus influenzae is a rare but challenging cause of PJI, often missed by conventional methods due to its fastidious nature.

Observation:

A 52-year-old male with rheumatoid arthritis on immunosuppressants developed PJI after hip arthroplasty.
Standard cultures were negative, but syndromic multiplex PCR (BioFire® JI panel) rapidly detected H. influenzae.
The patient was treated with debridement, antibiotic, and implant retention (DAIR), with targeted ceftriaxone therapy based on PCR results.

Findings:

Syndromic multiplex PCR provided rapid (2-hour) identification of H. influenzae.
Early pathogen detection enabled timely and targeted antibiotic therapy, de-escalating from broad-spectrum agents.
The patient experienced clinical recovery, with symptom resolution and implant retention.

Implications:

Rapid molecular diagnostics are crucial for identifying rare pathogens like H. influenzae in PJIs.
Timely diagnosis and targeted therapy improve clinical outcomes, reduce hospital stays, and preserve prosthetic implants.
This approach enhances antimicrobial stewardship and patient management in complex orthopedic infections.