Recombinant NK1 Protein and LEDs: An Innovative Strategy to Counteract Resistant Staphylococcus pseudintermedius and...

Area of Science:

• Microbiology
• Biotechnology
• Antimicrobial Research

Background:

• Multi-drug-resistant bacterial strains pose a significant global health challenge.
• There is a critical need for novel, sustainable, non-antibiotic therapeutic strategies.
• Staphylococcus pseudintermedius and Pseudomonas aeruginosa are key pathogens requiring new treatment approaches.

Purpose of the Study:

• To investigate a non-antibiotic approach combining recombinant NK1 protein and Light-Emitting Diodes (LEDs).
• To evaluate the antimicrobial and anti-virulence efficacy against resistant Staphylococcus pseudintermedius and Pseudomonas aeruginosa.
• To assess the combined effects on planktonic and sessile bacterial phases.

Main Methods:

• Recombinant NK1 protein produced via Pichia pastoris expression system.
• Antimicrobial activity assessed using Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC).
• Anti-virulence effects evaluated through motility assays, twitching assays, and anti-biofilm formation tests.

Main Results:

• Recombinant NK1 and LEDs demonstrated individual and synergistic antimicrobial and anti-virulence effects.
• Combined NK1 and LEDs achieved up to 85.98% CFU/ml reduction after 24h.
• Significant reduction in P. aeruginosa motility (up to 60%) and potent anti-biofilm activity observed.

Conclusions:

• The combination of recombinant NK1 protein and LEDs presents an innovative non-antibiotic strategy.
• This approach effectively inhibits the growth and virulence of Staphylococcus pseudintermedius and Pseudomonas aeruginosa.
• The findings support the development of eco-sustainable alternatives to traditional antibiotics.