Related Experiment Video
Updated: Sep 11, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
Published on: February 8, 2014
Enhanced three-dimensional imaging with large depth of field using pre-processing in optical scanning holography
Abstract:
Optical scanning holography (OSH) emerges as a groundbreaking single-pixel real-time holographic recording technique, charting new territory beyond conventional digital holography. A notable challenge in OSH, as with all three-dimensional (3D) imaging methods, is the susceptibility of reconstructed images corrupted by defocus noise. The noise is an unintended result of diffraction on the focused plane from out-of-focus planes of the three-dimensional object, which can degrade image quality upon holographic reconstruction. Current methods, however, all use some algorithms to post-process holographic data to achieve the purpose of eliminating out-of-focus noise, which is difficult to meet the requirements of real-time image processing. We propose an innovative pre-processing approach in optical scanning holography that not only eliminates defocus noise but also significantly enhances image quality. Preprocessing is accomplished by the utilization of a focus-tunable lens (FTL) to refocus the optical scanning beam according to the depth of the occlusion-free object. Complex holograms are then captured without the artifacts associated with defocus through real-time holographic data pre-processing. Numerical simulations and experimental results demonstrate that our proposed method can directly obtain high-quality reconstructed images with all the 3D information being in focus simultaneously.
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy
Three-Dimensional Microscopy in Microbiology
Super-resolution Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Overview of Microscopy Techniques

