A simple, cost-effective, method for creating electronic cigarette vapor condensate

Methods to create electronic cigarette (e-cigarette) vapor condensate are needed for use in e-cigarette vapor exposure studies. There are currently several methods to produce condensate described in the literature, but they are often cost-prohibitive, complex, or potentially hazardous, thus limiting the true availability of these methods to many researchers in the field. Here, we developed a method to make e-cigarette vapor condensate utilizing a button-activated vaping device and inexpensive supplies such as a syringe, vinyl tubing of varying diameters, an assortment of fittings, a conical tube, and ordinary, hard-sided, ice packs. The method of condensate production described here produced a yield of 35 µL of condensate per 15 puffs of e-cigarette vapor. This method is cost-effective, easy to perform, and can be readily used by researchers at a wide variety of institutions.